effect of temperates on beetroot pigment
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My independent variable will be the temperature of the distilled water.
My dependent variable will be the amount of beetroot pigment in the distilled water.
To test that the amount of pigment given off from the beetroot will increase with the temperature of water, I will put a piece of beetroot in a boiling tube filled with 200ml of distilled water at a defined temperature. The temperatures I will use will be 10c, 20c, 30c, 40c and 50c for each temperature I will repeat the experiment 3 times to make sure there are no anomalous results. This will also improve the accuracy of the results obtained.
To conduct the experiment I will first take a beetroot and a size 10 cork borer, with the cork borer I will bore a cylinder of beetroot and cut it to a length of 3cm. I will then ensure the distilled water pH level is constant by applying a buffer solution of pH 7, I will use 1 buffer tablet per 100ml of water and stir it twice. I will then put the boiling tube in an electric water bath or in a beaker filled with ice in order to get it to the required temperature, leaving a thermometer in so when the temperature is reached, I will be able to add the beetroot at the correct time. Once I have added the beetroot I will start a stopwatch and time it for 5 minutes. After the 5 minutes is up, I will remove the boiling tube and I will take a sample of 3ml from the water from inside and add it to a cuvette to for use in the colourimeter...