effect of Different Concentrations of amylase on starch
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The Effect of Different Concentrations of Amylase on Starch
The action of an enzyme in speeding the biochemical conversion of a substance into something else can be simply described as follows:
Enzyme
Substrate Product
In this experiment, amylase is the enzyme, and starch is the substrate, eg the substance it acts upon. Using different concentrations of amylase solution ranging from 1% to 2%, it will be added to a blue colour solution being iodine mixed to starch. The results should show the enzyme digesting the substrate and a reading will be taken of the time it takes for the amylase to fully breakdown the starch. This rate of activity can be measured by the apparent loss of blue colouring using the colorimeter.
Method
Using 6 test tubes labelled A to F, add 5cm of starch
Add sufficient iodine to just colour the solution
Add .1 cm of 1% amylase into the test tube A, and immediately place the contents into a curette and place into the colorimeter.
Take transmission readings every 30 seconds until the readings remain the same or after 5 minutes.
Repeat the process with amylase solution 1.25% into tube B, 1.5% into tube C, 1...