Relevance and usefulness of PCR technology and Hardy Weinberg law in identifying individuals
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INTRODUCTION:
In this lab we are looking at the relevance and usefulness of PCR technology and Hardy-Weinberg law in identifying individuals. The polymerase chain reaction (PCR) is an in vitro method for the primer-directed enzymatic synthesis of millions of copies of a specific DNA segment.(Meyers,1995) This reaction is based on the annealing and extension by a thermostable DNA polymerase of two oligonucleotide primers that flank the target DNA segment. The DNA is then denatured and each primer hybridizes to one of the two separated strands such that extension from the 3"S end toward the other. DNA polymerase extends the primers onto the template strand. (Meyers,1995) The new strands were then electrophoresed on DNA sequencing gels. These gels are usually fixed, dried and then processed for autoradiography. (Weber and May,1989) PCR technology plays an important role in the field of Forensic science. Conventional genetic markers like blood group serologic types or protein electrophoretic variants cannot be applied to many forms of forensic evidence. PCR on the other hand is capable of analyzing minute quantities of degraded DNA...