Temperature Effect on Trypsin
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How can Temperature affect the activity of Trypsin?
Hypothesis:
I predict that as the temperature increases the rate of the activity of trypsin will increase; only until the optimum temperature (30C - 40C) and after the optimum temperature the rate of the activity of trypsin will decrease (41C - 50C).
Aim:
This experiment will determine the effect of temperature on the activity of the enzyme trypsin which catalyses the breakdown of proteins to polypeptides. One way of increasing the rate of many chemical reactions is to increase the energy of the reactants by heating them.
Plan & Background Knowledge:
In this experiment we will measure the rate of the effect of trypsin in different temperatures. Most human enzymes have an optimum temperature of around 40C. By keeping our body at about 37C, we ensure that enzymes-catalysed reactions occur at close to their maximum rate. Therefore the range of temperature in which I will be investigating will be between 25 C and 50 C. The rate of the enzyme trypsin which catalyses the breakdown of proteins will increase if the temperature increases to the optimum temperature. As the casein is milky the time taken for the solution to become clear will be measured...