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Dehydrogenase - Strips Hydrogen atoms from organic molecules to reduce NAD
Decarboxylase - Br
Aim
The aim of this investigation is to find the type of sugar substrate yeast will respire most quickly.
The Respiration of yeast involves the use of the enzymes dehydrogenase and decarboxylase, as well as ATP (Adenosine Tri Phosphate - A living organisms quickest source of energy) synthase and acetyl CoA. ...
Amount/Concerntration of Yeast- The levels of yeast in the solution will give different sized results. More yeast will react more quickly, according to collision theory, which will give larger results, but if the reaction is too quick, the results may be too large for the measuring equipment we have avaliable, and will need more substrate to fuel its more vigorous reaction at a constant rate. Less yeast will react slower, but will give results that are closer together, and less accurate. ... It also defines the volume of the solution once made, an so affects the concerntration of yeast present. ...
Type of Yeast - The rate of reaction of different yeast types is different. Bakers yeast has been bred to create more CO2 as it respires, creating far better measurements, whereas brewers yeast is bred to make as much alcohol as possible, so the reate of reaction will appear slower.
Lag Phase - When dried yeast is used, the yeast must be allowed time to hydrate before it can react at full rate.
Over Time - As the yeast respires the substrate in the solution, the rate of reaction will appear to lower, as there is less substrate left to react, and so collision theory deffines that the reaction should slow. ...
Respiration
The respiration of yeast can be aerobic or anerobic. ... Once the sugars are taken in by the yeast, it is then respired to make ATP. ...
Preliminary Work
Equipment & Justification
Water Bath - Used to heat water, in which the yeast/sugar solution can be suspended, thus keeping the solution at a constant temperature. ...
Scales - High accuracy scales, allowing precise amounts of yeast to be added.
Safeyt Equipment - Overcoat to be worn to provent splashes of yeast and sugar substrate getting on me
Retort Stand - Used to hold things such as the boiling tube and gas collection tube. ... Now measure into the scales 1 gram of yeast, as accuratley as possible. ...
5) Re weigh the weighing boat, and you should now get the weight of any yeast left in the boat. This value can be subtracted from the first reading taken to get the exact amount of yeast in the boiling tube.
Approximate Word count = 2036
Approximate Pages = 8.1
(250 words per page double spaced)
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