123 School Work

culturin micro organisms

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  Aim:
  
  I will be culturing samples of known bacteria using standard procedures. The bacteria I will be culturing is Escherichia-coli (e-coli). After incubation I will note the appearance of growing colonies. This will give me experience in aseptic techniques. The appearance of the agar plates will indicate if I have been successful in excluding contamination by other micro-organisms.
  
  Time:
  
  I will need about 1 hour in the laboratory to set up the agar plates and, 48 hours later, about 30 minutes to examine them.
  
  Equipment and Materials:
  
       Sterile equipment: 1 petri dish containing nutrient agar; 2 dropper pipettes
       Sterile water
       Overnight broth cultures of micro-organism (Escherichia coli)
       Inoculation loop
       Glass spreader and foil covered beaker of 70% industrial methylated spirit for flame sterilisation
       Bunsen burner
       Discard pot containing disinfectant
       Marker pen
       Tape to secure Petri dishes
       Incubator at 25-30 C
  
  
  
  
  
  Safety and risk assessment:
  
       Wear protective clothing and eye protection.
       Do not open the Petri dishes after incubation. (BIOHAZARD)
  
  BIOHAZARD IS SOMETHING HAZARDOUS TO THE ENVIRONMENT AND TO THE LIVING CREATURES SUCH AS HUMANS.
  
       70% industrial methylated spirit...