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To begin my unknown research, I prepared and inoculated, with unknown #24, 2 NA slants, 1 NA plate and boiled and cooled 1 slant of FTM. ... First stain that was conducted was the Gram stain, which determined that the unknown was Gram negative with rods (Fig. ... This was done to verify the organism was, indeed rods. I also observed that there seemed to be a clear capsule around the organism. At this time I preformed the capsule stain, which did indeed verify that the organism was encapsulated. ... Growth was seen along the stab line and on top of the medium, but the organism did not liquefy the gel. ...
· MRVP-D: Residue seen on bottom tube, color hazy yellow
· Added 3% Hydrogen peroxide to NA Slant inoculated with unknown, this indicated catalase positive. ... Positive result indicates that this organism can use citrate as its sole source of carbon. ... Indicates organism is acidic. ... On the 2nd of April, two weeks after receiving the unknown, I prepared two transfer slants from the reserve slant, as well as a wet mount to test for motility. ... From the above results I had determined from my lab manual that the unknown #24 was a citrobacter.
Approximate Word count = 913
Approximate Pages = 3.7
(250 words per page double spaced)
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