effects of the temperature change on permeability of beetroot cell membrane
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If the temperature is too high, it will denature the protein molecues of the membrant that will destroy the selective permeability of th emembrane. The large molecules that are previously prevented to pass thorugh the membrane can now pass through them freely. Thus the pigment in the beetroot cells can diffuse out.
Alcohol is a strong dehydrating agent that will extract water from the cell. dehydration will destryo the colloidal nature of the protein molecules of the membrane and destroy their structure.
Volume of distilled water used; if more distilled water were to be used in some boiling tubes, the solution that was then obtained at the end would be more dilute and so would allow a higher percentage of light through. However, if less distilled water were to be used in some boiling tubes, then the solution that was obtained would be more concentrated and so would allow a lower percentage of light to pass through.
The percentage of light that is able to pass through the solutions of distilled water and pigment, which has leaked out of the beetroot samples.
Temperature of the environments.
The length/width of the beetroot samples...