123 School Work

Investigation To find the effect of a changing substrate concentration on the rate of an enzyme

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  Plan
  
  Aim
  
  The aim of this investigation is to find the effect of a changing substrate concentration on the rate of an enzyme-controlled reaction. We are going to look at the reaction of potato catalyse with a substrate concentration with amounts of hydrogen peroxide and water, which are going to be varied and changed all throughout the experiment.
  
  Preliminary Work
  
  The effect on pH on catalyse. We investigated the rate of which catalyse decomposed Hydrogen Peroxide at p H 3.
  
       Number of bubbles in five minutes
  Ph 3               
  Ph 4     125     117     122
  Ph 5     24     21     25
  
  The optimum Ph of catalyses is Ph7.
  
  
  
  Background Information & Scientific Explanation
  
  This is the reaction, which will take place in the experiment.
  Hydrogen Peroxide Hydrogen Peroxide Water + Oxygen
  2H2O2      2H2O +O2
  The Hydrogen Peroxide will decompose into water and oxygen.
  
  
  
  
  There are many factors that bring together substrate molecules and speed up
  
  There are many factors that bring together substrate molecules and speed up the rate of a reaction, they are:
       Enzyme concentration
       Substrate concentration so there are more particles present, increasing the chance of collisions occuring
       Temperature so that particles move around more rapidly, so there is a greater chance of collisions, converting substrate to product
       Ph
       Increase in pressure this will push the particles closer together
       Increase in surface area this will increase the chance of collisions
  
  Collision: When a hydrogen peroxide molecule collides with a potato catalayse, it fits into the enzymes active sites, it does so in such a way that old bonds are broken and new ones are formed, so that H202 (Substrate) in converted to H20 + O2 (product).
  Concentration: Up to a point the rate of reaction increases along with the concentration and Hydrogen Peroxide substrate concentration. However a point is reached when no further increase is possible, this is the maximum rate and is reached when all enzyme molecules are working at a full rate, there will be more particles present, which increases the chance of collisions, therefore speeding up the rate of reaction...